Thioglycolate Fluid Medium is a standard medium formulated and recommended by the European Pharmacopoeia, USP, APHA and FDA. The reducing agents thioglycolate and L-Cystine ensure anaerobiosis which is adequate even for fastidious anaerobes. The -SH groups of these substances also inactivate arsenic, mercury and other heavy metal compounds. Thioglycolate media are thus suitable for the examination of materials which contain heavy metals or heavy metal preservatives.
In the present formulation a special agar with a high viscosity but a very low turbidity is used. A very slow cooling is recommended to prevent stratification. The higher viscosity of the fluid thioglycolate medium prevents rapid uptake of oxygen. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes colour to pink..
Catalogue: DSHB3086
DEHYDRATED MEDIUM
- Colour: Beige
- Appearance: Hygroscopic powder
- Granulometry: 0.6 - 0.45 mm
- Additives: None
PREPARED MEDIUM
- Concentration : 30 g/L
- Clarity/Colour: Transparent / yellowish Pink-Violet Upper Layer
- Deposit: None
- Gel strength: Complies
- pH after sterilization: 6.90 - 7.30 at 25 °C
- Sterilization: by autoclaving at 121 °C for 15 minutes
Quantity: 500g
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